ZooKeys | 139: 1-32 (2023) A peer-reviewed open-access journal doi: 10.3897/zookeys.1 139.8164 1 RESEARCH ARTICLE #ZooKey S https:/ / ZOO keys. pensoft.net Launched to accelerate biodiversity research Tadpoles of four sympatric megophryinid frogs (Anura, Megophryidae, Megophryinae) from Mangshan in southern China Tianyu Qian'’, Yonghui Li?, Jun Chen’, Pipeng Li’, Daode Yang! | Institute of Wildlife Conservation, Central South University of Forestry and Technology, Changsha 410004, China 2. Institute of Herpetology, Shenyang Normal University, Shenyang 110034, China 3 Administration Bureau of Hunan Mangshan National Nature Reserve, Chenzhou 423000, Hunan, China Corresponding author: Daode Yang (csfuyydd@126.com) Academic editor: Annemarie Ohler | Received 30 March 2022 | Accepted 17 December 2022 | Published 9 January 2023 https://z00bank. org/DCAED79B-A88 1-4720-A549-DA889EE6CIDA Citation: Qian T, Li Y, Chen J, Li P Yang D (2023) Tadpoles of four sympatric megophryinid frogs (Anura, Megophryidae, Megophryinae) from Mangshan in southern China. ZooKeys 1139: 1-32. https://doi.org/10.3897/zookeys.1139.81641 Abstract Sympatric distribution and potentially long larval development time make the assignment of tadpoles confusing in Asian-horned frogs of the subfamily Megophryinae. In this study, we used molecular data to identify four syntopic megophryinid tadpoles from Mangshan on the border between Hunan and Guangdong provinces in southern China: Brachytarsophrys popei, Boulenophrys shimentaina, Bo. cf. om- brophila, and Bo. nanlingensis. A detailed re-description of the Br popei tadpoles is provided as well as the first descriptions of three Boulenophrys tadpoles based on external morphology and coloration. An effort is attempted to distinguish these tadpoles by coloration patterns: the dorsal pattern, ventral pattern, and pattern on tail are useful for field identification of these tadpoles. However, the variation of color pattern could sometimes make species delineation difficult. Researchers are encouraged to document coloration in life with photographs and the collection of tadpoles of different development stages and sizes advocated in order to better understand how color may change during larval development. Keywords Amphibian, integrative taxonomy, larvae, Megophrys, Nanling Mountains Copyright Tianyu Qian et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 2 Tianyu Qian et al. / ZooKeys 1139: 1-32 (2023) Introduction The Asian Horned Frogs in the subfamily Megophryinae Bonaparte, 1850 belong to seven genera and 129 recognized species with wide distributed in Asia, ranging from India and Bhutan to China and south to the Sundas and the Philippines (Frost 2022). Tadpoles of Megophryinae are characterized by a funnel-like oral disc, which allows them to feed beneath the water surface or anchor to a substrate for keeping safe during floods or other threats; this mouthpart specialization had intrigued scientists for almost a century (Hora 1928; Pope 1931; Liu 1950; Huang et al. 1991; Zeng 2021). Seven monophyletic clades were found within Megophryinae based on molecular analysis (Mahony et al. 2017), previously regarded as seven subgenera of Megophrys sen- su lato: Atympanophrys Tian & Hu, 1983; Boulenophrys Fei, Ye and Jiang in Fei & Ye, 2016 (under the name Panophrys Rao & Yang, 1997); Brachytarsophrys Tian & Hu, 1983; Megophrys Kuhl & Van Hasselt, 1822; Ophryophryne Boulenger, 1903; Pelobatrachus Bed- dard, 1908; and Xenophrys Giinther, 1864. Recent taxonomic studies have elevated the seven subgenera to the level of genera (Li et al. 2020; Lyu et al. 2021; Qi et al. 2021). However, the morphological differences remain insufficient for clear delineation of these genera. A recent review of the genus Brachytarsophrys by Li et al. (2020) regarded the tadpole ventral pattern as a diagnostic character for species identification. Subsequently, Tapley et al. (2020a) tentatively suggested the presence of “white longitudinal stripe on the ventrolateral surface of the head and body” in tadpoles as a diagnostic character for Brachytarsophrys. However, there are no known larval characters that differentiate the re- maining megophryinid genera from one another. The external morphology, including oral disc, as well as internal buccal features are highly conserved in all species in this subfamily (Huang et al. 1991; Grosjean 2003). The coloration in life, although rarely described, is considered useful for species identification since Leong and Chou (1998) and has attract- ed more and more attention (Oberhummer et al. 2014; Poyarkov et al. 2017; Tapley et al. 2017, 2020a, 2020b; Li et al. 2018; Munir et al. 2019; Liu et al. 2020; Shi et al. 2020a, 2020b, 2021; Wu et al. 2020). However, compared to the rate of new megophryinid frog species descriptions, tadpole descriptions remain scarce or are very brief, resulting in insuf- ficient diagnostic characters for comparison and/or field recognition. Before molecular analysis was widely used in megophryinid taxonomic studies, tad- poles were assigned to species based on their association with post-metamorphic/adult specimens collected from the same site, e.g., Boulenophrys jinggangensis (Wang in Wang et al. 2012), Xenophrys mangshanensis (Fei & Ye in Fei et al. 1990), tadpoles described by Fei and Ye (2016) and Xenophrys longipes (Boulenger, 1886), tadpoles described by Leong and Chou (1998). Notably, many megophryinid species are reported to be in sympatry, including sister species (e.g., Wang et al. 2014, 2019; Chen et al. 2017; Poyarkov et al. 2017; Liu et al. 2018; Mahony et al. 2018, 2020; Shi et al. 2020a; Tapley et al. 2018, 2020b, 2021). Some megophryinid tadpoles are suspected of having long larval devel- opment periods of over one year (Grosjean 2003; Tapley et al. 2020a). Consequently, cf. tadpoles collected from the same site with adult frogs may not necessarily belong to the same species. Thus, the description of tadpoles without molecular data has become suspicious if there are any other megophyinid species discovered in sympatry. Tadpoles of four megophryinid frogs from China 3 Mangshan, a part of Nanling Mountains, is located on the border between Hunan and Guangdong provinces in southern China. The first megophryinid record from this area was a tadpole with a funnel-like oral disc collected at the mountain top of Guang- dong/Hunan border (Mell 1922), which was identified as “Megalophrys boettgeri” (cur- rently Boulenophrys boettgeri). This record has been shown to be erroneous as no adult B. boettgeri frogs were found in this area (Shen 1983; Shen et al. 2014), and the horned frogs collected in Mangshan were identified as “Megophrys kuatunensis” (currently Bo. kuatunensis), “Megophrys brachykolos’ (currently Bo. brachykolos), and Brachytar- sophrys carinense in “Fauna Hunan, Amphibia” (Shen et al. 2014). Subsequently, this “Brachytarsophrys carinense” population was described as a new species Brachytarsophrys popei Wang, Yang & Zhao in Zhao et al. (2014), while the identity of the two Boule- nophrys frogs, that were both previously regarded as widespread (e.g., Fei et al. 2009; Fei and Ye 2016) was questioned after molecular analysis based on large-scale sampling (Tapley et al. 2017; Liu et al. 2018; Gao et al. 2022). In this study, we collected tadpoles bearing funnel-like oral disc from Mangshan. Using DNA barcoding, we confirmed that this collection of syntopic tadpoles was com- posed of four species: Brachytarsophrys popei, Boulenophrys nanlingensis (Lyu, Wang, Liu & Wang in Wang et al. 2019), Bo. shimentaina (Lyu, Liu & Wang in Lyu et al. 2020), and Bo. cf. ombrophila (Messenger & Dahn in Messenger et al. 2019). Based on the ex- amination of external morphology and coloration in life, we re-described the tadpoles of Br. popei and provided the first description of the three Boulenophrys tadpoles. Materials and methods Samples All tadpoles were collected from Mangshan, Yizhang, Hunan Province, China during field surveys in June, July, and November 2021. Specimens were photographed in life in a transparent acrylic box before being euthanized with buffered tricaine methane- sulfonate (MS-222) and then fixed with 10% formalin for storage. Tissue samples (tail fin/muscle) were preserved in 95% ethanol for molecular analysis. Specimens were de- posited at the Institute of Wildlife Conservation, Central South University of Forestry and Technology (CSUFT), Changsha, China. Molecular analysis Segments of the 16S ribosomal RNA gene (16S) were used for species identification. Primer sequences (L3975 and H4551) from Simon et al. (1994) were used for PCR amplification in 50 pl reaction volumes under the following conditions: 98 °C for 2 min; followed by 30 cycles of 98 °C for 10 sec, 55 °C for 10 sec, and 72 °C for 10 sec, with a final extension step at 72 °C for 5 min. PCR purification and sequencing were performed by Biomarker Technologies Co. (Beijing, China). The new sequenc- es were then searched on BLAST (NCBI) to verify their approximate identity. The 4 Tianyu Qian et al. / ZooKeys 1139: 1-32 (2023) identification of GenBank accession numbers were retrieved from the BLAST result and manually verified by checking the original references. Uncorrected p—distance be- tween the new sequences and sequences from the BLAST result was calculated using MEGA 6 (Tamura et al. 2013). Before calculating uncorrected p—distances, sequences were aligned using the MUSCLE algorithm with default parameters (Edgar 2004) and trimmed with gaps partially deleted in MEGA 6. Morphology Image J 1.53k software (Schneider et al. 2012) was used to measure the tadpoles from photographs of preserved specimens taken next to a scale (10 mm length). The stag- ing follows Gosner (1960), and the terminology for external morphology follows Altig and McDiarmid (1999). Measurements and morphometric abbreviations follow Oberhummer et al. (2014). Definitions of abbreviations are as follows: BH body height, maximal body height at trunk; BL body length from snout to the point where the axis of the tail myotomes meets the body wall; BS body end to the center of spiracle; BW maximal body width; ED eye diameter; ES eye—snout distance; IND the internarial distance measured from center to center; IOD the interorbital distance measured from center to center; MTH maximal tail height; LFH lower fin height at MTH; UFH upper fin height at MTH; NE distance from the center of naris to the center of the eye; ODW oral disc width; SN distance from the center of naris to snout; SS distance from snout to the center of spiracle; TTL total length; TAL tail length = TTL - BL; TMH tail muscle height at the body-tail junction, where ventral line of muscula- ture meets trunk contour; TMW tail muscle width at the same level as TMH. For the measurement of oral disc width (ODW) in preserved specimens, we ex- panded the oral disc by anchoring it to a glass (as shown in Fig. 3D). We compared the tadpoles with their congeneric tadpoles described in the litera- ture where molecular data has been used to confirm species identity: Br popei, tad- poles described by Zhao et al. (2014) and Li et al. (2020); Br. feae (Boulenger, 1886), Br. orientalis Li, Lyu, Wang & Wang in Li et al. 2020, and Br. chuannanensis Fei, Ye & Tadpoles of four megophryinid frogs from China 5 Huang in Fei and Ye 2001, tadpoles described by Li et al. (2020); Br. intermedia (Smith, 1921), tadpoles described by Tapley et al. (2020a); Bo. lini (Wang & Yang in Wang et al. 2014); Bo. fansipanensis (Tapley, Cutajar, Mahony, Nguyen, Dau, Luong, Le, Nguyen, Nguyen, Portway, Luong & Rowley, 2018), Bo. hoanglienensis (Tapley, Cuta- jar, Mahony, Nguyen, Dau, Luong, Le, Nguyen, Nguyen, Portway, Luong & Rowley, 2018), and Bo. jingdongensis (Fei & Ye in Fei et al. 1983), tadpoles described by Tapley et al (2020b); Bo. baishanzuensis (Wu, Li, Liu, Wang & Wu, 2020); Bo. lushuiensis (Shi, Li, Zhu, Jiang, Jiang & Wang, 2021); Bo. rubrimera (Tapley, Cutajar, Mahony, Chung, Dau, Nguyen, Luong & Rowley, 2017); Bo. jiangi (Liu, Li, Wei, Xu, Cheng, Wang & Wu, 2020); and Bo. leishanensis (Li, Xu, Liu, Jiang, Wei & Wang, 2018). We further summarized the morphological characteristics of all megophryinid tadpoles that were identified based on molecular data as described in the literature: Atympano- phrys gigantica (Liu, Hu & Yang, 1960), tadpoles described by Tapley et al. (2020b); Ophryophryne elfina Poyarkov, Duong, Orlov, Gogoleva, Vassilieva, Nguyen, Nguyen, Nguyen, Che & Mahony, 2017; Pelobatrachus kalimantanensis (Munir, Hamidy, Mat- sui, Iskandar, Sidik & Shimada, 2019); Xenophrys medogensis (Fei, Ye & Huang, 1983), X. cf. pachyproctus (Huang in Huang and Fei 1981), and X. yeae (Shi, Zhang, Xie, Jiang, Liu, Ding, Luan & Wang, 2020), tadpoles described by Shi et al. (2020a); X. maosonensis (Bourret, 1937), tadpoles described by Tapley et al. (2020b); X. serchhipii Mathew & Sen, 2007, tadpoles described by Raj et al. (2022); X. lekaguli (Stuart, Chuaynkern, Chan-ard & Inger, 2006); “X. katabhako” Deuti, Grosjean, Nicolas, Vas- udevan & Ohler, 2017 (synonymized to X. monticola Giinther, 1864 by Mahony et al. 2018); X. periosa (Mahony, Kamei, Teeling & Biju, 2018), tadpoles described by Shi et al. (2020b); and “Megophrys” dringi Inger, Stuebing & Tan, 1995, tadpoles described by Oberhummer et al. (2014). Results Tadpole identification Two tadpoles were identified as Brachytarsophrys popei based on an uncorrected p— distance of 0.0-0.7% from the samples in the type series from Hunan, Guangdong, and Jiangxi Provinces (GenBank accession numbers: KM50425 1-KM504258). Three tadpoles from the same collection site and bearing the same characteristics as the above two tadpoles but without molecular data were also assigned to Br. popei. The collection site of these tadpoles is only ~ 5 km from the collection site of paratype SYS a00589 (GenBank accession number: KM504051) in the adjacent Nanling Nature Reserve, Guangdong Province. A total of 14 tadpoles was identified as Boulenophrys nanlingensis, which exhibited an uncorrected p—distance of 0.0—0.4% from the holotype SYS a001964 (GenBank accession number: MH406646) collected ~ 10 km in the adjacent Nanling Nature Reserve, Guangdong Province. 6 Tianyu Qian et al. / ZooKeys 1139: 1-32 (2023) Five tadpoles were identified as Boulenophrys shimentaina, which exhibited an uncorrected p—distance of 1.2% from the type series (GenBank accession numbers: MH406655—MH406656, and 787-788) collected from Shimentai Nature Reserve, Guangdong Province, China ~ 70 km to the south of Mangshan. This genetic distance is smaller than the interspecies p—distance in the subfamily Megophryinae used to identify tadpoles (1.4% in Br. intermedia, Tapley et al. 2020a). Four tadpoles showed an uncorrected p—distance of 1.8—2.1% from the type se- ries of Bo. ombrophila (GenBank accession numbers: KX856397—KX856404) collected from Wuyishan, Fujian Province, China ~ 500 km to the northeast of Mangshan. ‘This genetic distance almost equals to the threshold (2.0% in 16S gene, proposed by Chen et al. 2017) for a potential new species in this subfamily. However, the other two popula- tions of Bo. ombrophila suspected by Messenger et al. (2019) from Jiulianshan, Jiangxi Province (Megophrys sp 8 in Liu et al. 2018) and Renhua County, Guangdong Province (M. sp 9 in Liu et al. 2018) were closer to our samples both in the geographical distance of collection sites and genetic distances based on 16S gene. The Jiulianshan population of Bo. ombrophila (GenBank accession numbers: MH406836—-MH406840) collected from ~ 160 km to the east of Mangshan showed an uncorrected p—distance of 1.6—-1.8% from our samples. The Renhua population of Bo. ombrophila (GenBank accession num- bers: MH406650-MH406653, and MH406834) collected from ~ 70 km to the east of Mangshan showed an uncorrected p—distance of 1.4—1.6% from our samples. Further examination of adult frogs revealed morphometric differences between the Mangshan population and the type series from Wuyishan, Fujian. However, the morphometric and morphological data were unavailable for the Jiulianshan population and Renhua Popu- lation. Thus, we currently identified the Mangshan population as Bo. cf. ombrophila. Morphological description All examined specimens exhibited a funnel-like oral disc corresponding to the tadpole description of “Megophrys minor” by Liu (1950): “The mouth is terminal, with a large funnel that has two long lateral wings, a short ventral wing and a comparatively nar- row convex flap above. ‘The tips of the lateral and ventral wings are bluntly pointed.” Detailed tadpole descriptions are given below. Brachytarsophrys popei Fig. 1 Remark. The following description is based on five tadpoles at Stages 26—27 (N = 2) and 36-37 (NV = 3). Body ratio ranges represent all specimens. Raw measurements are given in Table 1. Specimens examined. CSUFT T10115 (Stage 37, Field voucher: MT05; GenBank accession number: ON209276), CSUFT T10117 (Stage 37; Field voucher: MT07; GenBank accession number: ON209284), and CSUFT T10119 (Stage 36; Tadpoles of four megophryinid frogs from China 7 Figure |. Brachytarsophrys popei tadpoles in life A-C tadpole CSUFT T10117 (Stage 37) lateral view, dorsal view, and ventral view D ventral pattern of tadpole CSUFT T10119 (Stage 36) E ventral pattern of tadpole CSUFT T10945 (Stage 26); and F oral disc of tadpole CSUFT T10117 (Stage 37). D and E share the same scale bar with A-C. Field voucher: MT09; not sequenced), collected on 30 May 2021 from Tiantaishan (24.972277°N, 112.963394°E, ca. 1280 maz.s.l.), Mangshan, Hunan Province, China; and CSUFT 110944 (Stage 27, Field voucher: MT1104; not sequenced), and CSUFT T10945 (Stage 26; Field voucher: MT1105; not sequenced), collected on 16 Novem- ber 2021 from the same site as the first specimens. External morphology. ‘The body is oval, robust, and flattened above (BW/BL 53.3— 55.7% at Stages 26-27, N = 2; and 53.6-55.2% at Stages 36-37, NV = 3); the head is wid- er than the trunk; the eyes are located dorsolaterally, the pupils are round; the nares are oval, opening laterally, closer to the eye than to the tip of the snout (NE/SN 68.8-73.3% at Stages 26-27, N = 2; and 73.7—83.3% at Stages 36-37, N = 3); the internarial distance 8 Tianyu Qian et al. / ZooKeys 1139: 1-32 (2023) Table |. Morphometric data of the tadpole specimens used in this study. For abbreviations, see Materials and Methods. “*” indicates specimens with broken tails, and “\” indicates “no data”. Species Voucher No. v 4 T ™ tr QQ 3 — Tow AXA a Zw 3 FE se £228 e E RHE OzzZ & # zB 8 Brachytarsophrys. CSUFTT10944 27 318 9.0 228 42 50 42 12 30 54 17 19 38 2.5 11 16 2.7 2.7 48 54 popes CSUFTT10945 26 27.9 7.9 20.0 3.9 49 34 12 2.9 52 14 16 38 2.6 1.1 15 26 25 44 5.9 CSUFTTI10115 37 35.0 10.5 245 47 61 45 14 31 64 21 20 46 28 14 19 33 29 58 7A CSUFTTI10117 37 37.3 11.0 263 5.0 63 47 15 29 58 19 19 47 28 15 20 34 29 59 7.8 CSUFTT10119 36 36.9 10.9 260 5.0 63 49 14 32 63 21 21 47 3.0 15 18 32 3.1 59 7.6 Boulenophrys CSUFTT10156 25 285 7.3 21.2 31 3.9 38 08 23 44 12 11 3.1 22 10 14 23 24 40 52 shimentaina CSUFTT10277 26 286 8.0 206 38 46 35 10 25 45 13 13 34 24 10 16 26 24 44 63 CSUPT’ 110279" +25" \ 83 1. 37 4739 10-26. \ 4 N° .34 23°1o 15 24.25 43 66 CSUPT'T10285. 27°:28:5. 81 204.3% 47 36°10°°28" 56 15. 15° 34.23 10° 1S 24 Q4@4a 57 CSUFTT10283 28 27.0 80 190 3.5 44 37 0.9 26 48 13 12 32 23 09 14 23 22 41 60 Boulenophrys CSUFTT10144 25 187 54 13.3 23 3.2 2.5 0.7 17 27 07 08 23 16 07 10 17 15 28 3.8 nanlingensis CSUFTT10986 35 40.1 10.8 29.3 54 64 45 16 37 73 17 16 47 32 15 24 37 36 57 78 CSUFTT10969 34 344 88 25.6 40 56 42 13 3.0 55 14 13 3.9 27 11 19 30 28 48 5.6 CSUFTT10261 25 25.1 67 184 3.1 42 2.7 08 18 40 12 12 2.7 19 08 12 20 15 38 54 CSUFTT10262 25 27.2 65 20.7 28 40 28 10 19 42 12 12 29 19 09 12 21 17 36 52 CSUFTT10273 28 35.7 94 263 44 54 43 11 32 59 15 15 40 28 10 18 29 3.0 5.0 7.9 CSUFTT10991 27 39.1 10.3 288 44 60 44 13 31 67 20 18 40 28 12 19 3.1 28 53 7.6 CSUFTT10284 25 189 5.3 136 26 3.3 22 08 17 34 10 09 26 16 08 10 18 16 32 41 CSUFT T10302* 25 \ 73 \ 33 40 33 09 24 \ \ \ 30 20 09 13 22 21 40. 6.0 CSUFTT10303 25 26.2 68 194 3.3 3.9 3.1 08 23 40 13 12 29 19 09 13 22 20 39 49 CSUFTT10377 27 28.1 82 19.9 35 49 34 10 23 53 15 15 34 23 09 16 24 20 44 68 CSUPT 110378 28.269" 89 187 Si4--5.0°3.2- 12 245 52 D3 14 19:4 220-09) 14-95 792 42 O59 CSUFTT10376 27 248 7.0 178 35 41 31 10 21 48 14 14 31 21 09 13 21 20 40 57 CSUPTT10370: 29: “378 7 207) 3:5" 45.9340 12 25~50. 13714-9422 10. 14°23 9 AO. 57 Boulenophrys cf. CSUFT'T10270 36 33.7 10.0 23.7 45 5.6 46 14 29 60 14 16 42 28 14 17 3.0 29 5.1 83 ombrophila GSUFTT10272 27° 33.1 -8:9° =24.2--41. 5,3. 42 12. 3.0 GOT 14 14°38 25 11 7 28 28 48 7.8 CSUFTT10288 26 304 84 22.0 39 48 38 Ll 27 59 16 14 36 24 11 15 26 26 46 72 CSUFTT10992 25 209 5.1 15.8 2.1 28 2.0 0.5 16 3.0 08 08 2.2 15 06 10 17 13 27 3.9 is smaller than the interorbital distance IND/IOD 65.8-68.4% at Stages 26-27, N = 2; and 59.6—63.8% at Stages 36-37, N = 3); the rims of nares are raised from the body wall and directed posterolaterally; the spiracle is sinistral and low on the left flank; the spira- cle tube is short, protruding posterodorsally, free from the body at the tip, and opening posterolaterally (SS/BL 55.6-62.0% at Stages 26-27, N = 2; and 57.3-58.1% at Stages 36-37, N = 3); the anal tube opens medially, unattached to the ventral fin; the dorsal fin arises behind the body-tail junction while the ventral fin is connected to the trunk; the tail muscle is massive, taller than tail fins before reaching the maximum tail height (TMH/MTH 55.6-55.8% at Stages 26-27, N = 2; and 48.4—50.8% at Stages 36-37, N = 3), and the tail tip is bluntly pointed, the tail length accounts for 71.7% (at Stages 26-27, N = 2) and 70.5—70.5% (at Stages 36-37, N = 3) of the total length; the mouth is terminal and the oral disc is funnel-like (BW/ODW 74.6-88.9% at Stages 26-27, N = 2; and 75.6—78.4% at Stages 36-37, N = 3); three and four rows of short oval submarginal papillae can be observed on the upper lip and lower lip, respectively; keratodonts are ab- sent; the upper jaw sheath is brush-like, exhibiting a small median notch, while the lower jaw sheath is thin, sickle-shaped, weakly keratinized, and finely serrated. Tadpoles of four megophryinid frogs from China ) Coloration. In life, the background color of the head and trunk is dark brown; the dorsal pattern is pale brown interspersed with dark brown chromocytes, extend- ing to above the horizontal level of the spiracle on the trunk from a lateral perspec- tive; the dorsal surface of the anterior part of the tail is pale brown marbled with dark brown speckles; neuromasts are distinctly visible on the head, trunk and tail; the region between the anterior edges of the eyes and the median point of the upper lip is pigmented with a dark brown V-shaped pattern; the narial rims are pale brown; the oral disc is golden-pigmented, with a translucent edge; the submarginal papillae on lips are dark brown-pigmented. Laterally, the tail is pale brown-pigmented; dense goldish spots are located at the anterior part of the lateral surface of tail muscle, becoming smaller and at the middle, then disappearing posteriorly; three distinct dark brown stripes extended from the body-tail junction, and horizontally positioned at the anterior part of the tail; the upper and lower stripes end before reaching the maximum tail height, while the middle stripe is about half the length of the others; the upper and middle stripes are incomplete; the anterior part of the upper fin is opaque, marbled with goldish pigmentation and brown speckles; the anterior part of the ventral fin, as well as the anal tube are semi-translucent with dense large golden spots; the rest of the fins are semi-translucent, and exhibit sparse dark brown speckles interspersed with small goldish dots. The ventral surface of the body is rather dark; the belly is dark purplish covered with dense white spots; two longitudinal stripes, positioned ventrolaterally, extending from the snout to the vertical edge of the eyes posteriorly, and sometimes appear to broken; a transverse bar is positioned at the head-trunk junction of the vertical edge of the anterior spiracle and is always inter- rupted at the middle; the spiracle region and the corresponding region on the other side of the body, are covered with a short white stripe, that starts from the head-body connection, and terminated before reaching the region of the spiracle tube opening; regions without white pigmentation have less melanocytes; the gills and gut coils are indistinctly visible through the ventral skin. The eye sclera is silver with black dots; the iris periphery is wide and black; the iris is golden sprinkled with black dots; and the spiracle is translucent without pigmentation. In tadpoles at Stages 36-37, the hindlimbs are semi-transparent, and the outer aspect of the legs exhibits brown pig- mentation interspersed with goldish chromocytes. In preserved specimens, the tail stripes are still prominent; an incomplete V-shaped pigmentation pattern is still visible; the ventral pattern is translucent milky white; the golden pigmentation wanes on the oral disc; and the hindlimb bones are visible in ventral view in Stage 36-37 tadpoles. Comparisons. Tadpoles of Br. popei differ significantly from the three syntopic Boulenophrys tadpoles described below by the unique pattern of two longitudinal white ventrolateal stripes on head, a transverse white bar on chest, and distinct large spots on belly (vs. absence of stripes and bars, and smaller spots/speckles on belly). The differences in ventral pattern between four Brachytarsophrys tadpoles were compared by Li et al. (2020) and summarized in Table 2. The tadpole of Br. popei (Stage 29, NV = 1) illustrated in their paper (also in Zhao et al. 2014, but marked as 10 Tianyu Qian et al. / ZooKeys 1139: 1-32 (2023) Table 2. Comparison of color pattern among tadpoles of the subfamily Megophryinae which were iden- tified based on molecular data. ‘ CD in the figure, and “\” indicates “no data”. ’ indicates characteristics not mentioned in the text but were illustrated Species References Atympanophrys A, gigantica Tapley et al. 2020b Brachytarsophrys Br. popei 26-27, and distinct | indistinct} uniform dark small dots and ventrolateral stripes on This study 36-37, N=5 brown longtitudinal stripes | head and body, incomplete transverse bar on chest, dense large spots on belly 26-29, N= 14 three dark two longitudinal white Zhao et al. longitudinal stripes stripes along the sides of 2014 body, a completed transverse bar on chest, belly mottled with dense white speckles Br. intermedia pale brown not pale brown with speckled with ventrolateral stripes on head | ‘Tapley et al. visible a darker brown dark brown, and and body, small spots on 2020a medial saddle longitudinal stripes chest and belly Br. \ distinct dark wide ventrolateral stripes on | Li et al. 2020 chuannanensis longtitudinal stripes* | head*; wide transverse bar on chest; and several spots on belly* Br. orientalis three short dark —_| two short, longitudinal white | Li et al. 2020 longitudinal stripes stripes on sides of ventral surface of head and body; absence of transversal white stripe on chest; belly mottled with dense white speckles Br. feae transeverse bar on chest; } Liet al. 2020 several several transeverse stripes on belly Boulenophrys Bo. shimentaina distinct brown with dark | pigmented with dense} milky white ventrolateral This study brown reticulation | dark brown markings spots on chest, dense posteriorly indistinct small milky white speckles on belly Bo. cf. 25, N= indistinct | distinct pale brown, pigmented orange belly covered with dense ‘This study ombrophila 1 (TTL scattered with and dark brown melanocytes 20.9 mm) dense dark speckles melanocytes 26-27, and distinct brown pattern several large brown gold-pigmented white This study 36, N=3 along mid-vertical spots along tail ventrolateral spots on chest, (TTL 30.4— line muscle dense white speckles on belly 33.1 mm) Bo. nanlingensis | 25, N=2 distinct distinct | yellowish with pale | many brown speckles gold-pigmented white This study (TTL 18.7— orangish blotches, ventrolateral spots on chest, 18.9 mm) or brown with sparse white speckles on belly whitish patterns 25, N=3 distinct distinct | pale brown with | many brown speckles gold-pigmented white ‘This study (TTL 25.1- dark brown ventrolateral spots on chest, 27.2 mm) pigmentation sparse white speckles on belly 27-29, N= distinct distinct | bi-colored dorsum | many brown speckles gold-pigmented white This study 4, TTL 24.8— of pale brown ventrolateral spots on chest, 28.1 mm) anteriorly and dark sparse white speckles on belly brown posteriorly 27-28, and distinct distinct | uniform brownish | many brown speckles gold-pigmented white This study 34-35, N=4, ventrolateral spots on chest, (TTL 35.7— sparse white speckles or 44.4 mm) dense large spots on belly Bo. fansipanensis| 25, N=2 obvious visible | brown with dark | small spots and dark | a translucent grey brown and| ‘This study brown speckles brown speckles speckled with metallic blue and flecked with dark brown 11 Tadpoles of four megophryinid frogs from China Species Stage Neuromasts | Intestine | Dorsum pattern Pattern on tail Ventral pattern References visibility | visibility Bo. jingdongensis\| 25, N=1 indistinct | visible | dark brown with many dark brown grey brown and speckled | Tapley et al. cream blotches, speckles with metallic blue 2020b bordered by orange flecks Bo. distinct dark brown with many dark brown | speckled with metallic grey | Tapley et al. hoanglienensis reddish brown speckles blue flecks 2020b blotches and reticulated blackish brown Bo. rubrimera obvious brown with darker | pale yellowish brown | speckled white and brown | ‘Tapley et al. speckles with speckles 2017 Bo. brownish black | small white and black Wa et al. baishanzuensis spots 2020 Bo. lushuiensis 26-27, 32, visible brown without pale brown with scattered with silver tiny | Shi et al. 2021 and 36, N=5 distinct patterns | dozens of small dark patches brown patches Bo. leishanensis | 25-26, N=6 | _ visible* yellow-brown pale colored on fins, | dense small white speckles* | Li et al. 2018 and small black spots on tail muscle Bo. jiangi yellow-brown few dark spots on \ Liu et al. posterior tail muscle* 2020 Ophryophryne O. elfina 25,N=5 visible not uniform brownish | few round blackish pale brownish orange, Poyarkov et al. visible red or brownish spots on tail intestine 2017 orange Pelobatrachus PR 30, and 36, visible* not conspicuous dark | marbled with dark | belly milky-white pigmented, | Munir et al. kalimantanensis N=2 visible* | brown and gold | brown pigmentation, | _ pale stripe below spiracle 2019 or orange brown edges of fins with extends laterally to half of pigmentation golden iridophores abdomen* 45, N=1 invisible* not dark brown dark brown dark brown marbled pattern | Munir et al. visible* without orange 2019 gold pigmentation Xenophrys X. medogensis 35, and 38, \ \ pale yellow-brown | mottled with pale without white patches Shi et al. (low-elevation) N=2 colored patches 2020a X. medogensis deep brown brown, scattered with | semitransparent brown, Shi et al. (high-elevation) with copper tiny white pigment covered with small white 2020a pigmentation _| spots, no dark brown pigments patches on tail X. cf. yellow-brown with \ Shi et al. pachyproctus two golden spots 2020a on dorsalateral mid body X. yede 28-29, and brown with dense above lower fin semi-transparent Shi et al. 31-35, N=9 copper pigments | mottled with copper 2020a patches X. maosonensis 25, N=2 obvious visible | brown with dark few dark brown speckled with metallic Tapley et al. brown speckles speckles grey blue 2020b posteriorly X. lekaguli 25, 37-38, pale gray (in proximal half of small black spots (in Stuart et al. and 42, N=6 preservative) caudal muscle with preservative) 2006 two or three irregular dark streaks, fins distinctly pigmented only in distal portions (in preservative) X. serchhipii dark brown (in | translucent and grey | dark brown, fins are opaque | Raj et al. 2022 preservative) (in preservative) | and speckled (in preservative) X. monticola grey olive-green densely arranged immaculate, slightly Deuti et al. with irregular melanophores (in translucent with some rare 2017 melanophores (in preservative) preservative) spots of melanophores (in preservative) 12 Tianyu Qian et al. / ZooKeys 1139: 1-32 (2023) Species Stage Neuromasts | Intestine | Dorsum pattern Pattern on tail Ventral pattern References visibility | visibility X. periosa 27, N=1 greyish brown dense small speckles | translucent greyish brown Shi et al. 2020b 34, N=1 \ \ greyish brown large spots alongside | translucent greyish brown Shi et al. anterior 2/3 of tail 2020b muscle Incertae sedis with Megophryinae “Megophrys” 25, N=4 \ visible conspicuous pigmented dark | milky translucent with a few | Oberhummer dringi pattern of intense | brown, interspersed | irregularly shaped golden et al. 2014 dark brown and with pale golden spots gold pigmentation iridophores Stage 27), which was collected ~ 200 km north of Mangshan has a complete transverse white ventral bar. In contrast, our tadpoles (Stages 26-27, N = 2; and Stages 36-37, N = 3) consistently exhibit an interrupted white transverse ventral bar. This difference may be due to geographic variation or insufficient sample size. However, the presence of a transverse bar on chest could distinguish Br popei tadpoles from Br. orientalis and Br. intermedia (vs. absent in both). In addition, the width of the transverse bar is markedly smaller than that in Br. chuannanensis (see Li et al. 2020: fig. 5E, F). Furthermore, compared with Br. intermedia, the tadpoles of Br. popei have a distinctly smaller size at Stage 36 (TTL 36.9 mm vs. 48.7 mm). Zhao et al. (2014) illustrated a metamorph of Br. feae at Stage 44 with several short stripes on belly (vs. spots or speckles in Br. popei, Br. orientalis, and Br. chuannanensis). We believe this pattern should be confirmed using more specimens at an earlier developmental stage in case this is a transitional form during metamorphosis. Further comparisons between By popei tadpoles and all megophryinid tadpoles that were identified using molecular data are shown in Tables 2 and 3. Ecology notes. All tadpoles were collected from an artificial roadside drainage ditch (Fig. 5C) at night while feeding beneath the water surface. Upstream of the ditch is a narrow, slow-moving stream with many rocks covered by moss. The ditch was rocky with a sandy substrate. The maximum depth of this ditch was ~ 0.5 m. Branches of plants from the mountain side of the road extended over this ditch, however, sun- light did reach the water surface at certain times of the day. Tadpoles were observed in a still water stretch behind big rocks, or a small dam formed by submerged leaf litter. Three tadpoles at Stages 36-37 were collected on 30 May 2021 at 22:30 h, together with tadpoles of Bo. shimentaina and Bo. nanlingensis with an ambient air temperature of ~ 20 °C. Two tadpoles at Stages 2627 were collected on 16 November 2021 at 19:30 h with an ambient temperature of ~ 13 °C. Tadpoles were considered nocturnal because we did not encounter any tadpoles during the day. Male Br. popei frogs began their calling activities under rock crevices in this ditch in late July. Zhao et al. (2014) reported that the breeding season of Br. popei is July to September, and that their tadpoles (Stages 26—29) were collected in April and December. This indicates that the development of these tadpoles may be very prolonged, and it is likely that they can over winter. Interestingly, it is unknown why no tadpoles were collected during the breed- ing season both in this study and in Zhao et al. (2014). Tadpoles of four megophryinid frogs from China 13 Table 3. Comparison of morphological characteristics among tadpoles of the subfamily Megophryinae, which was identified based on molecular data. > 99 were illustrated in the figure, and “\” indicates “no data”. indicates characteristics not mentioned in the text but (expanded) shape Atympanophrys A, gigantica 35 5 50.7 (42.6—| 16.9 (15.7— | 66.6 (63.2—| 62.6, N= 1 hastate serrated | broadly Tapley et al. 54.9) 18.0) 68.4) and raised | rounded 2020b Brachytarsophrys Br. popei 26-27 36.441.2 | 8.5+0.8 71L7(-) | 81.7£10.1 | bi-triangular bluntly ‘This study (35.0-37.3) | (7.9-9.0) (74.6-88.9) pointed 36-37 36.4£1.2 | 10.840.3 | 70.340.3 | 77.24£1.4 | bitriangular bluntly This study (35.0-37.3) | (10.5—11.0) | (70.0-70.5) | (75.6-78.4) pointed 26-27 bluntly | Zhao et al. 2014 29 bluntly | Zhao et al. 2014 Br. intermedia 32 14.0£2.2 | 69.7£1.7 Tapley et al. (12.4-15.5) | (67.9-70.3) 2020a 36 bi-triangular Tapley et al. 2020a 39 bi-triangular Tapley et al. 2020a Br. orientalis 36 Liet al. 2020 Boulenophrys Bo. fansipanensis | 25 30.8 (26.5— bi-triangular | serrated | pointed Tapley et al. 35.0) and raised 2020b Bo. jingdongensis | 25 27.9 bi-triangular | serrated | rounded | Tapley et al. and raised 2020b Bo. hoanglienensis| 26 26.5 79.3, N=1| bi-triangular | serrated | pointed Tapley et al. and raised 2020b Bo. shimentaina | 25-27 28.5£0.1 72.7£1.5 | 72.3+5.8 | bi-triangular | serrated | bluntly This study (28.5—28.6) (71.6-74.4) | (65.2-77.2) and raised | pointed 28 27 8 70.4 68.3 bi-triangular | serrated ‘This study and raised | pointed Bo. cf. 25 1 75.6 69.2 bi-triangular | serrated | bluntly This study ombrophila and raised | rounded 26-27 31.8£1.9 | 8740.4 | 72.7£0.5 | 62.741.7 | bitriangular | serrated | sharply This study (30.4-33.1) | (8.4-8.9) | (72.4—73.1) | (61.5-63.9) and raised | pointed 33.7 10.0 bi-triangular | serrated This study and raised | pointed Bo. nanlingensis 26.046.4 | 7.1£1.5 bi-triangular | serrated This study (18.7-39.1) | (5.3-10.3) and raised 30.144.8 bi-triangular | serrated | pointed ‘This study (26.9-35.7) and raised 34.4 bi-triangular | serrated This study and raised 40.1 bi-triangular | serrated | pointed This study and raised | Bo. lushuiensis | 26-27 27. 8£4.0 \ Shi et al. 2021 (23.1-30.3) 32 42.7 12.1 71.9 Shi et al. 2021 36 1 41.1 11.3 72.5 58.4 \ \ \ Shi et al. 2021 Bo. rubrimera 37 33.3 10.5 68.5 Tapley et al. 2017 \ Bo. pointed | Wuetal. 2020 baishanzuensis Bo. leishanensis 29.7423 64.2+2.1 Liet al. 2018 (27.0-33.0) (61.5-66.7) Bo. jiangi i Liu et al. 2020 Bo. lini Wang et al. 2014 not provided pointed pointed pointed Wang et al. 2014 14 Tianyu Qian et al. / ZooKeys 1139: 1-32 (2023) Species References Ophryophryne O. elfina 25 “nares Poyarkov et al. tubular” | rounded 2017 Pelobatrachus P kalimantanensis| 30 Munir et al. 2019 Munir et al. 2019 Munir et al. 2019 Xenophrys X, yeae 28-29 34.3+0.4 10.6+0.3 Shi et al. 2020a (33.9-34.8) | (10.2—-11.0) 31-34 34.9+1.1 11.0+0.5 Shi et al. 2020a (33.7-35.8) | (10.4-11.4) 3) 1 38.4 10.9 rounded*} Shi et al. 2020a X. of. 25 bluntly | Shi et al. 2020a pachyproctus pointed* X. medogensis 27 pointed* | Shi et al. 2020a (high-elevation) X. medogensis 35 pointed* | Shi et al. 2020a (low-middle 38 Shi et al. 2020a elevation) X. maosonensis 25 35.5 (34.4-| 8.8 (8.1- narrowly | —Tapley et al. 36.6) 9.5) rounded 2020b X. lekaguli 25 9.0-10.4 \ \ not raised | rounded | Stuart et al. 2006 ay 12.1-12.9 \ \ not raised | rounded | Stuart et al. 2006 38 1 \ \ \ not raised | rounded | Stuart et al. 2006 42 1 \ \ \ not raised | rounded | Stuart et al. 2006 X. serchhipii 32 1 5. \ \ \ Raj et al. 2022 34 4 10.2+0.30 \ \ \ \ Raj et al. 2022 elevated projection” 37 28.9 11.9 58.8 \ Raj et al. 2022 38 1 \ \ \ \ Raj et al. 2022 X. monticola 25 24.7£2.7 \ \ “waves” finely | Deuti et al. 2017 (21.1- rounded. 28.1), N X. periosa 27 8.9+0.1 bi-triangular* \ bluntly | Shi et al. 2020b (8.4-9.5) (58.2-64.5) pointed 34 3) 47 3+4.4 12.8+0.9 72.941.1 75.8£5.9 | bi-triangular* \ bluntly | Shi et al. 2020b (42.7—-51.4) | (12.1-13.8) | (71.7-73.9) | (69.9-81.6) pointed Incertae sedis with Megophryinae “Megophrys” 25 32.28+46.05| 9.1141.89 raised and Oberhummer et dringi (23.23- (6.74— (69-73) projected al. 2014 37.63) 11.35) Boulenophrys shimentaina Fig. 2 Remark. ‘The following description is based on five tadpoles at Stages 25—28 (NV = 5). Body ratio ranges represent all specimens. Raw measurements are given in Table 1. Specimens examined. CSUFT T10156 (Stage 25; Field voucher: MT06; Gen- Bank accession number: ON209270) collected on 30 May 2021 from Tiantaishan (24.972277°N, 112.963394°E, ca. 1280 maz.s.l.), Mangshan, Hunan Province, China; Tadpoles of four megophryinid frogs from China 15 and CSUFT 110277 (Stage 26, Field voucher: MT707; GenBank accession number ON209281), CSUFT T10279 (Stage 26; Field voucher: MT709; GenBank accession number: ON209264), CSUFT T10283 (Stage 28, Field voucher: MT713; GenBank accession number: ON209261); and CSUFT T10285 (Stage 27; Field voucher: MT715; GenBank accession number: ON209272) collected on 14 July 2021 from Xiangsikeng (24.937705°N, 112.990257°E, ca. 1530 m, a.s.l.), Mangshan, Hunan Province, China. External morphology. The body is oval and flattened above (BW/BL 51.3- 55.0%, N = 5); the eyes are located dorsolaterally, and the pupils are round; the nares are oval, open laterally, closer to the eye than to the tip of the snout (NE/SN 62.5-71.4%, IND/IOD 67.6—71.9%, N = 5); the rims of nares are serrated, slightly raised from the body wall; the spiracle is sinistral, low on the left flank; the spiracle tube is short, free from the body at the tip and opens laterally (SS/BL 53.4-58.0%, N = 5); the anal tube opens medially, unattached to the ventral fin; the dorsal fin arises behind the body-tail junction while the ventral fin is connected to the trunk; the tail muscle is massive, taller than tail fins before reaching the 2/3 part of the tail length (TMH/MTH 50.0-55.6%, NV = 5); the tail tip is bluntly pointed, the tail length accounts for 69.5—76.1% (NV = 4) of the total length; the mouth is terminal and the oral disc is funnel-like (BW/ODW 65.2-—77.2%, N = 5); four rows of oval submarginal papillae are visible on the upper lip, and five rows of oval submarginal papillae on the lower lip; keratodonts are absent; the upper jaw sheath is comb-like, exhibiting a small median notch; the lower jaw sheath is thin and sickle-shaped, weakly keratinized, and finely serrated. Coloration. The following description is based on a tadpole at Stage 27 (CSUFT T10285). In life, the background color of the body and tail is semi-transparent dark brown; the dorsum is pigmented pale brown which extends to the dorsal surface of anterior tail and gradually becomes golden; a distinct circled marking is present at the center of dorsum, forming a saddle with the background dark brownish coloration; the middle of the saddle is pigmented pale brown; and the neuromasts are distinctly visible. Laterally, the dorsal pattern extends to the region above the horizontal level of the spiracle on the trunk, and covers the whole lateral surface of head; the lat- eral surface of tail is pigmented brown; the tail and fins are covered with irregularly shaped pale golden spots, interspersed with dense dark brown speckles; the fins are semi-transparent; the anterior part of the dorsal fin is marbled with golden and dark brown speckles; the junction of the anterior half of the dorsal fin and the caudal muscle is pigmented dark brown, forming an incomplete line; the anterior part of the ventral fin and the anal tube exhibit minimal dark brown pigmentation; the posterior part of tail and fins are pigmented with dense dark brown markings. The ventral body is semi- translucent grey, pigmented with dark brown chromocytes, and is covered with dense small, indistinct milky-white speckles; the gills and gut coils are visible through the ventral skin; two large, milky-white spots are present on each side of the ventrolateral surface of head-body connection and are followed by a cluster of smaller spots. The oral disc is translucent milky white; the lateral and middle wings are covered with orangish 16 Tianyu Qian et al. / ZooKeys 1139: 1-32 (2023) 10 mm Figure 2. Boulenophrys shimentaina tadpoles A=-C freshly dead tadpole CSUFT T10285 (Stage 27) lateral view, dorsal view, and ventral view D oral disc of tadpole CSUFT T10283 (Stage 28) in life E ventral pattern of tadpole CSUFT T10277 (Stage 26) in life; and F ventral pattern of CSUFT T10283 (Stage 28) in life. E and F share the same scale bar with A-C. pigmentation; the tips of the wings and the middle of the upper lip exhibit dark brown pigmentation; the submarginal papillae on lips are dark brown, and the narial rims are pigmented beige. The eye sclera is silver with black dots; the iris is orange sprinkled with black dots; and the spiracle is translucent without pigmentation. Variation of coloration in life. The other four tadpole specimens match most of the descriptions above. However, the dorsum pattern of a saddle is not clearly visible in CSUFT T10156 and the dorsum is almost uniform pale brown in CSUFT T10177. The ventrolateral spots on head-body connection are very large in CSUFT T10283 (Stage 28, Fig. 2F), but smaller in CSUFT T10277 (Stage 26, Fig. 2E). Tadpoles of four megophryinid frogs from China U7 In preserved specimens, the pale brown pigmentation on the dorsal surfaces of the body and tail are still visible; the golden and orangish pigmentation fade to milky white; the white spots on each side of the ventrolateral surface of head-body connection become translucent; there is no orange pigmentation on the mouthparts, and promi- nent black pigmentation can be observed on the tail. Comparisons. The two distinct, conspicuous ventrolateral spots on ventrolateral surface of head-body connection could distinguish the tadpoles of Bo. shimentaina from most Boulenophrys tadpoles, including Bo. fansipanensis, which have a single spot visible on each side, and Bo. rubrimera, Bo. hoanglienensis, Bo. jingdongensis, Bo. leis- hanensis, Bo. jiangi, and Bo. lushuiensis with no ventrolateral spots; the ventral pattern of indistinct, small speckles on belly could distinguish Bo. shimentaina tadpoles from Bo. lini, which have dense large speckles (see Wang et al. 2014: fig. 5F). Furthermore, the tadpoles of Bo. shimentaina differs from Bo. lushuiensis by having a silver sclera with black dots (vs. black with golden pigments); and from Bo. baishanzuensis by having a pale brown pattern on dorsum (vs. uniformly brownish black). Tadpoles of Bo. shimentaina could be distinguished from the syntopic Boule- nophrys tadpoles in Mangshan (see below for the descriptions) by having a dark brown background coloration of body and tail (vs. pale brown in Bo. cf. ombrophila and Bo. nanlingensis), and a tail pattern of dense dark brown markings posteriorly (vs. several large brown spots along tail muscle in Bo. cf. ombrophila; and many brown speckles in Bo. nanlingensis). Further comparisons between Bo. shimentaina tadpoles and all megophryinid tadpoles identified based on molecular data are shown in Tables 2, 3. Ecology notes. A single tadpole at Stage 25 was collected on 30 May 2021, to- gether with the tadpoles of Bo. nanlingensis and Br. popei from the road ditch (Fig. 5C) that was mentioned above in the Br. popei section. Four tadpoles at Stages 25—28 were collected together with tadpoles of Bo. nanlingensis and Bo. cf. ombrophila from a rocky, slow-flowing narrow stream (Fig. 5B) on 14 July 2021 at 23:20 h while nearby adult males were calling. As this stream is located near the mountain top, it is narrow and slow. There were low trees and bamboo on both sides of the stream, and many fallen logs lay across the stream with a rocky stream bed. This site was used by many species as a breeding site including Bo. nanlingensis, Bo. shimentaina, Br. popei, Leptobrachella mangshanensis (Hou, Zhang, Hu, Li, Shi, Chen, Mo & Wang, 2018), and Quasipaa exilispinosa (Liu & Hu, 1975). The tadpoles of Bo. shimentaina found in this stream were observed at night in an area with sandy substrate near the stream bank or in still water behind a small dam formed by submerged leaf litter. Sunlight could reach the surface of these areas at certain times during the day. While feeding beneath the water surface, the tadpoles rely on submerged leaf litter or rocks (Fig. 5A). Once disturbed, they hid quickly under the submerged leaf litter and emerged from the leaf litter af- ter several seconds. In the still water area where these tadpoles were found, we also encountered many Q. exilispinosa tadpoles on the stream substrate, and a subadult newts, Pachytriton xanthospilos Wu, Wang & Hanken, 2012, hiding under submerged leaf litter. Male Bo. shimentaina frogs were observed calling from late June to August in Mangshan, and it was suggested that the breeding season of Bo. shimentaina is from 18 Tianyu Qian et al. / ZooKeys 1139: 1-32 (2023) April to August in Shimentai Nature Reserve, Guangdong Province (Lyu et al. 2020). It is not clear if tadpoles complete metamorphosis within a single year, and we didn't collect any tadpoles of more advanced developmental stages. Boulenophrys cf. ombrophila Fig. 3 Remark. ‘The following description is based on four tadpoles at Stages 25—27 (N = 3) and Stage 36 (NV = 1). Body ratio ranges represent all specimens except where specified. Raw measurements are given in Table 1. Specimens examined. CSUFT 110992 (Stage 25; field voucher: MT02; Gen- Bank accession number: ON209283) collected on 3 June 2021; and CSUFT T10281 (Stage 26; field voucher: MT718; GenBank accession number: ON209275), CSUFT 10270 (Stage 36, field voucher: MT710; GenBank accession number: ON209267), and CSUFT 110272 (Stage 27, field voucher: MT712; GenBank accession number: ON209269) collected on 14 July 2021. All specimens were collected from Xiang- sikeng (24.937705°N, 112.990257°E, ca. 1530 m, a.s.l.), Mangshan, Hunan Prov- ince, China. External morphology. The body is flattened and oval (BW/BL 52.9-54.8% at Stages 25-27, N = 3; and 51.0% at Stage 36, NV = 1); the eyes are located dorsolater- ally, the pupils are round; the nares are oval, opening laterally, closer to the eye than to the tip of the snout (NE/SN 60.0—73.3% at Stages 25-27, N = 3; and 82.4% at Stage 36, N = 1); the internarial distance is smaller than interorbital distance (IND/IOD 65.8-68.2% at Stages 25-27, N = 3; and 66.7% at Stage 36, N = 1); the rims of nares are serrated, slightly raised from the body wall; the spiracle is sinistral, low on the left flank, and opens posterolaterally; the spiracle tube protrudes posteriorly, free from the body at the tip (SS/BL 54.9-59.6% at Stages 25-27, N = 3; and 56.0% at Stage 36, N = 1); the anal tube opens medially, unattached to the ventral fin; the dorsal fin arises behind the body-tail junction while the ventral fin is connected to the trunk; the tail muscle is massive, taller than tail fins until reaching 2/3 of the tail length (TMH/MTH 45.8-53.3% at Stages 25-27, N = 3; and 48.3% at Stage 36, N = 1); the tail tip is usu- ally sharply pointed (bluntly rounded in one small-sized specimen CSUFT T10992, Stage 25, TTL 20.9 mm); the tail length accounts for 72.4—75.6% of the total length at Stages 25-27 (NV = 3), and 70.3% at Stage 36 (V = 1); the mouth is terminal and the oral disc is funnel-like (BW/ODW 61.5-69.2% at Stages 25-27, N = 3; and 61.4% at Stage 36, NV = 1); three and four rows of short oval submarginal papillae are present on the upper and lower lips, respectively; keratodonts are absent; the upper jaw sheath is comb-like, exhibiting a small median notch, whereas the lower jaw sheath is thin and sickle-shaped, weakly keratinized, and finely serrated. Coloration. The following description is based on a tadpole at Stage 27 (CSUFT T10272, Fig. 3A—C). In life, the background color of the body and tail is semi-transpar- ent beige; the dorsal surface of the body is covered by a pale brown pattern that extends Tadpoles of four megophryinid frogs from China 19 D 1mm Figure 3. Boulenophrys cf. ombrophila tadpoles A—C tadpole CSUFT T10272 (Stage 27) lateral view, dorsal view, and ventral view in life D oral disc of tadpole CSUFT T10270 (Stage 36) in preservative E ventral pattern of tadpole CSUFT T10281 (Stage 26) in life; and F ventral pattern of tadpole CSUFT T10270 (Stage 36) in life. E and F share the same scale bar with A-C. to the dorsal surface of the anterior part of the tail; a dark spot is present between the eyes and followed by a short beige vertical line on the anterior dorsum; the neuromasts are distinctly visible; and sparse dark brown markings alongside the vertical line and the dorsolateral neuromasts. Laterally, the dorsal pattern extends to above the horizontal level of the spiracle; three large, whitish, and golden pigmented spots are present be- hind the eyes on each side of the lower part of head-body connection, two of them are visible from the ventral view; the lateral surface of the tail and fins is covered by irregu- larly shaped pale golden spots, interspersed with whitish chromocytes which form short lines, and brown chromocytes which gather into large spots along the tail muscle at the posterior part of the tail; the fins are semi-transparent; the anterior part of the dorsal fin 20 Tianyu Qian et al. / ZooKeys 1139: 1-32 (2023) is marbled with golden and dark brown speckles; the anterior part of the ventral fin and the anal tube exhibit minimal brown pigmentation whereas the rest of fins that exhibits sparse dark brown speckles. ‘The ventral body skin is translucent beige, covered by dense milky white speckles; the gills and gut coils are indistinctly visible through the ventral skin. The oral disc is translucent beige; the lateral and middle wings are covered by orange pigmentation; the submarginal papillae on lips are dark brown; the narial rims are yellow; the eye sclera is silver with black dots; the iris is bright orange sprinkled with black dots; the spiracle is translucent, with scattered golden pigmentation. Variation of coloration in life. Among the remaining three specimens examined, two tadpoles at Stages 26 and 36 match most of the coloration pattern of the descrip- tion for CSUFT T10272 above (see Fig. 3E, F for ventral patterns). However, the dark spot between the eyes is not present in both; the vertical line is more distinct in CSUFT T10281, which extends from the middle of the eyes to the body-tail connection; and the vertical line in CSUFT T10270 is a bit longer than CSUFT 110272, which extend to the posterior dorsum. In the Stage 36 tadpole (CSUFT T10270), the hindlimbs are semi-transparent, and the legs are covered externally by brown chromocytes. A small- sized tadpole at Stage 25 (CSUFT T10992, TTL 20.9 mm) exhibited a significantly different coloration from other three tadpoles of larger body size (Stages 26-27, and 36; TTL 30.4-33.7 mm): the dorsal pattern is pale brown, with scattered dense dark melanocytes, especially at the vertebral line region; an inconspicuous orange V-shaped pattern between the anterior edges of the eyes and the median point of the upper lip; two orangish spots at the posterior edge of the eyes; the orangish pigmentation is also diffuse on the dorsal aspect of the body and tail; the ventral skin is almost clear, trans- lucent milky, with sparse goldish speckles on the edge of the belly; the belly is covered with dense melanocytes, however, gut coils clearly visible through these melanocytes. In preserved specimens, a fading of the dorsal pattern is observed; the golden spots on the lateral surfaces of the tail are not visible; the large spots on the anterior corner of the spiracle and gills become translucent, and the orange pigmentation on the lips disappears. Comparisons. The tadpoles of Bo. cf. ombrophila differ from the syntopic tadpoles of Bo. shimentaina by the semi-translucent beige background coloration of body and tail (vs. dark brown), a ventral pattern of dense milky white speckles on belly (vs. indis- tinct small speckles), and the pattern on tail of large spots along tail muscle (vs. dense markings posteriorly); from Bo. nanlingensis (see below for tadpole description) by the ventral pattern of dense whitish speckles (vs. sparse speckles), and the pattern on tail of several large spots (vs. many speckles). Compared to other described Boulenophrys tadpoles where species identification is supported with molecular data, the tadpoles of Bo. cf. ombrophila differs by the ventral pattern of dense whitish speckles (vs. relatively sparse metallic blue speckles in Bo. fansipanensis; sparse whitish speckles in Bo. jingdongensis; sparse metallic grey blue speckles in Bo. hoanglieneneis; and scattered with silver tiny patches in Bo. lushuiensis), and the tail pattern of several large spots along tail muscle (vs. few dark brown speck- les in Bo. fansipanensis; absence of large spots in Bo. jingdongensis; many dark brown speckles in Bo. hoanglienensis; small black spots in Bo. baishanzuensis; few dark spots on Tadpoles of four megophryinid frogs from China 24 posterior tail muscle in Bo. jiangi; small black spots on tail muscle in Bo. leishanensis; and dozens of small dark brown patches in Bo. lushuiensis). Further comparisons be- tween Bo. cf. ombrophila tadpoles and all megophryinid tadpoles that were identified based on molecular data are shown in Tables 2, 3. Ecology notes. One observed breeding site of Bo. cf. ombrophila was a relatively broad wetland crossed by a small shallow creek. Several water sources from the gen- tle slope of the bamboo forest fed this creek and made the entire area very wet. ‘This breeding site was muddy, covered with leaf litter and fallen logs. The creek was narrow and slow flowing with maximum depth of 0.2 m. Some fallen logs blocked the creek and created still water areas. Only male Bo. cf. ombrophila and Q. exilispinosa were observed calling in this site during our visits from May to August, and in November. The potential predator of these frogs, an aquatic snake Opisthotropis cheni Zhao, 1999 which was observed once, in July, in this creek. A single small-sized tadpole specimen (CSUFT T10992, TTL 20.9 mm) at Stage 25 was collected from this site while the male frogs were heard calling before a heavy rainstorm on 3 June 2021 at 19:30 h at dusk. Three tadpoles were collected from the rocky area (Fig. 5B, mentioned above in the Bo. shimentaina section) 20 m downstream of this creek together with tadpoles of Bo. shimentaina and Bo. nanlingensis. Interestingly, male Bo. cf. ombrophila frogs were not observed calling in the rocky area, and the other two species did not breed in this wetland. This indicates a different microhabitat preference between these congeneric species. The breeding season of Bo. cf. ombrophila ends in mid-July in Mangshan. It is not clear if tadpoles will complete metamorphosis during the year. Boulenophrys nanlingensis Fig. 4 Remark. The following description is based on 14 tadpoles at Stages 25—29 (NV = 12), and 34-35 (N = 2). Body ratio ranges represent all specimens except where specified. Raw measurements are given in Table 1. Specimens examined. CSUFT 110144 (Stage 25; field voucher: MT04; GenBank accession number: ON209279) collected on 30 May 2021; and CSUFT T 10302 (Stage 25, field voucher: MT722; GenBank accession number: ON209280), and CSUFT T10303 (Stage 25, field voucher MT723; GenBank accession number: ON209277) collected on 19 July 2021 from Tiantaishan (24.972277°N, 112.963394°E, ca. 1280 ma.s.l.); CSUFT T10261 (Stage 25; field voucher: MT701; GenBank accession num- ber: ON209263), CSUFT T10262 (Stage 25; field voucher: MT702; GenBank ac- cession number: ON209268), CSUFT T10273 (Stage 28, field voucher: MT703, GenBank accession number: ON209278), CSUFT T10991 (Stage 27; field voucher: MT711; GenBank accession number: ON209265), and CSUFT T10284 (Stage 25, field voucher: MT714; GenBank accession number: ON209271) collected on 14 July 2021; and CSUFT 110986 (Stage 35, field voucher: MT1106; GenBank acces- sion number: ON209285) and CSUFT T10969 (Stage 34, field voucher: MT1109; 22 Tianyu Qian et al. / ZooKeys 1139: 1-32 (2023) GenBank accession number: ON209274) collected on 19 November, 2021 from Xiangsikeng (24.937705°N, 112.990257°E, ca. 1530 m, a.s.l.); and CSUFT T10376 (Stage 27, field voucher: MT756; GenBank accession number: ON209273), CSUFT T10377 (Stage 27, field voucher: MT757; GenBank accession number: ON209262), CSUFT 110378 (Stage 28, field voucher: MT758; GenBank accession number: ON209282), and CSUFT 110379 (Stage 29, field voucher: MT769; GenBank acces- sion number: ON209266) collected on 28 July 2021 from Guizizhai (24.952750°N, 112.960470°E, ca. 1210 ma.s.l.), Mangshan, Hunan Province, China. External morphology. The body is elongated, oval, and flattened above (BW/BL 51.2-60.4% at Stages 25-29, N = 11; and 52.8-54.5% at Stages 34-35, N = 2); the eyes are located dorsolaterally, and the pupils are round; the nares are oval, closer to the eye than to the tip of the snout (NE/SN 55.6-80.0% at Stages 25-29, N= 12; and 57.9-62.5% at Stages 34-35, N = 2); the internarial distance is smaller than interor- bital distance (IND/IOD 61.5—71.0% at Stages 25-29, N = 12; and 68.1-69.2% at Stages 34-35, NV = 2); the nares open laterally; the rims of nares are serrated, slightly raised from the body wall; the spiracle is sinistral, low on the left flank, and opens posteriorly; the spiracle tube is short and slightly protrudes posteriorly (SS/BL 54.8— 62.7% at Stages 25-29, N = 12; and 59.3-63.6% at Stages 34-35, N = 2). The anal tube opens medially and is unattached to the ventral fin; the dorsal fin arises behind the body-tail junction, and the ventral fin is connected to the trunk. The tail muscle is massive, deeper than tail fins before reaching the maximum tail height (TMH/MTH 43.4-63.0% at Stages 25-29, N = 11; and 50.7-54.5% at Stages 34-35, N = 2); the tail tip is pointed, the tail length accounts for 69.5—76.1% (at Stages 25-29, N = 11) and 73.1—-74.4% (at Stages 34-35, NV = 2) of the total length; the mouth is terminal and the oral disc is funnel-like (BW/ODW 63.3—79.6% at Stages 25-29, N = 12; and 73.1-85.7% at Stages 34-35, N = 2); four and five rows of short oval submarginal papillae can be observed on the upper and lower lips, respectively; keratodonts are absent; the upper jaw sheath is comb-like, exhibiting a weak median notch; the lower jaw sheath is thin and sickle-shaped, weakly keratinized, and finely serrated. Coloration. The following description is based on a tadpole at Stage 25 (CSUFT T10303, Fig. 4A—C). In life, the background color of the body and tail are semi- transparent grey; the dorsal surface of the body is covered by a pale brown pattern that extends to the dorsal surface of the anterior part of the tail; roughly symmetrical dark brown pigmentation can be observed on the dorsal body; and the neuromasts are distinctly visible. Laterally, the dorsal pattern extends to above the horizontal level of the spiracle; the lateral surface of the tail is pigmented brown, interspersed with pale golden spots and irregular dark brown speckles; the fins are semi-transparent and scat- tered with pale golden spots; the anterior part of the dorsal fin is marbled with golden and dark brown speckles, with the dark brown speckles forming an incomplete line; the anterior part of the ventral fin and the anal tube, lacks brown pigmentation but with sparse golden speckles. The ventral surface of the body is semi-transparent grey; the gills appear pink through the ventral skin; two large gold-pigmented white spots are present at ventrolateral head-body connection; the gut coils are distinctly visible Tadpoles of four megophryinid frogs from China 23 Figure 4. Boulenophrys nanlingensis tadpoles in life A-C tadpole CSUFT T10303 (Stage 25) lateral view, dorsal view, and ventral view D ventral pattern of tadpole CSUFT T10262 (Stage 25) E ventral pattern of tadpole CSUFT T10273 (Stage 28); and F oral disc of tadpole CSUFT T10261 (Stage 25). D and E share the same scale bar with A-C. through the ventral skin, the belly is scattered with small whitish speckles; the oral disc is translucent beige; the lateral and middle wings are covered by orangish pigmenta- tion; the submarginal papillae on lips are dark brown; the narial rims are beige; the eye sclera is silver with black dots; the iris is copper-colored sprinkled with black dots, comparable to the iris coloration in adults; and the spiracle is translucent. Variation of coloration in life. The dorsal pattern coloration in tadpoles of Bo. nanlingensis is subject to significant variation both in same stages and between stag- es. At Stage 25, a small-sized tadpole (CSUFT T10144, TTL 18.7 mm) exhibits a yellowish dorsum with pale orange blotches, and dark brown pigmentation present 24 Tianyu Qian et al. / ZooKeys 1139: 1-32 (2023) collected Bo. shimentaina tadpole (middle) feeding together with two unrecognized tadpoles in its habitat (B); and tadpole habitat (C) of a mixed-species assemblage beside a forest road, with unrecognized tad- poles feeding together (D). posteriorly; another small tadpole (CSUFT T10284, TTL 18.9 mm) displays a brown dorsum with whitish patterns on the dorsolateral surfaces of the trunk and these extend to the tip of the tail. The coloration of the medium-sized tadpoles at Stage 25 (CSUFT 710261, TTL 25.1 mm; and CSUFT 710262, TTL 27.2 mm) and a broken-tailed individual at Stage 25 (CSUFT T10302) correspond to the dorsal pat- tern of CSUFT T10303 described above. However, the shape and coverage of dark brown markings varies between individuals. At later Stages 27-29, three medium- sized tadpoles (CSUFT 10377, Stage 27, TTL 28.1 mm; CSUFT T10376, Stage 27, TTL 24.8 mm; and CSUFT 10378, Stage 28, TTL 26.9 mm) exhibit a bi-colored Tadpoles of four megophryinid frogs from China 25 dorsum, which is anteriorly pale brown and posteriorly inconspicuous dark brown. Tadpoles with relatively larger size at both early Stages 27-28 and advanced Stages 34-35 (CSUFT T10273, Stage 28, TTL 35.7 mm; CSUFT T10991, Stage 27, TTL 39.1 mm; CSUFT T10986, Stage 35, TTL 40.1 mm; and CSUFT T10969, Stage 34, TTL 34.4 mm) exhibit a uniform brownish dorsum coloration with almost invisible markings. Two tadpoles, CSUFT 110144 (Stage 25, TTL 18.7 mm) and CSUFT T10379 (Stage 29, TTL 27.8 mm) exhibit pale yellowish dorsum with orange pig- mentation, which are indistinguishable from the small-sized Bo. cf. ombrophila tadpole (CSUFT T10992, TTL 20.9 mm). A tadpole at Stage 27 (CSUFT T10376, TTL 24.8 mm) with a mid-vertical line on dorsum is similar with that of larger Bo. cf. ombrophila tadpoles (Stages 26-27, and 36; TTL 30.4-33.7 mm). However, they were not collect- ed form the same site as Bo. cf. ombrophila tadpoles. A large-sized tadpole at Stage 35 (CSUFT 10986, TTL 40.1 mm) showed a ventral pattern of large spots on belly that was different with other specimens. For tadpoles at Stages 34-35 (CSUFT T10969, and CSUFT T10986), the hindlimbs are semi-transparent, the outer aspect of the legs is pigmented yellow and interspersed with brown chromocytes on top. In preserved specimens, a fading of the dorsal pattern is observed; the tail is trans- lucent with sparse dark-brown pigmentation; the orange pigmentation on lips is no longer visible; the whitish speckle on the ventral surface and the nares are translucent. Comparisons. The variation of dorsum pattern makes the tadpoles of Bo. nanlin- gensis are sometimes confused with the syntopic tadpoles of Bo. cf. ombrophila. Usu- ally, the ventral pattern of sparse speckles (vs. dense speckles) and the tail pattern of many small speckles (vs. large spots) could distinguish them. An exception is the small- sized tadpole CSUFT 10144 (Stage 25, TTL 18.7 mm), which bears almost the same pattern as a small-sized Bo. cf. ombrophila tadpole CSUFT T10992 (Stage 25, TTL 20.9 mm). The tadpoles of Bo. nanlingensis differ from the syntopic Bo. shimentaina tadpoles by the pale brownish background coloration of the body and tail (vs. dark brown), the ventral pattern of sparse speckles (vs. dense small speckles), and the tail pattern of small dots (vs. large speckles). Compared to other described Boulenophrys tadpoles where species identification is supported by molecular data, the tadpoles of Bo. nanlingensis differs by the presence of ventrolateral spots on each side of head-body connection (vs. absent in Bo. jingdon- gensis, Bo. hoanglienensis, Bo. leishanensis, and Bo. lushuiensis); the tail pattern of many brown speckles (vs. small spots on tail muscle in Bo. leishanensis; few dark spots on pos- terior tail muscle in Bo. jiangi; and small white and black dots in Bo. baishanzuensis). Further comparisons between Bo. nanlingensis tadpoles and all megophryinid tadpoles that were identified based on molecular data are shown in Tables 2, 3. Ecology notes. ‘Tadpoles of Bo. nanlingensis were discovered in all collection sites during our field surveys in 2021, which perhaps implies that this species has larger popu- lation size, or it might exhibit less microhabitat specificity. Besides the three sites men- tioned above, four Bo. nanlingensis tadpoles were collected from a relatively wide stream (3-5 m wide), with a maximum depth of 0.5 m. An adult male was observed calling under rocks near the stream bank with its feet standing in shallow water on 28 July 2021. 26 Tianyu Qian et al. / ZooKeys 1139: 1-32 (2023) The male calling activities of Bo. nanlingensis, which began in late July, had in- creased during our visit in November in Mangshan. It seems the newborn larva would have to over-winter. Thus, we suspected the tadpoles of early Stages 25—29 collected in May and July were born in the previous year. Two tadpoles at advanced Stages 34—35 were collected on the 19" of November. Considering tadpoles in late stages develop relatively fast (Grosjean, 2003; TQ, personal observation). It was likely these advanced tadpoles would finish metamorphosis in cold season at the beginning of the next year. However, this assumption needs further confirmation because the cold weather and scarce food in winter may not be suitable for the survival of froglets. Discussion In this study, we attempted to identify sympatric megophryinid tadpoles using external morphology and color patterns, especially ventral patterns. However, our sample size was small. Tadpoles of Bo. shimentaina and Bo. cf. ombrophila bearing stable and dis- tinct ventral pattern were collected from a single site. It is not clear if the color pattern may differ between sites. In Bo. nanlingensis, the color pattern varied between different body size ranges rather than stages or collection sites. This provides new insight into that the coloration pattern perhaps should be classified by body size ranges in mego- phyinid tadpoles, and not only development stages. Diagnostic larval characters for delineating megophryid genera are still insufficient except for the ventrolateral pattern in Brachytarsophrys. However, there are characters shared within genera, such as the rim of nares is “tubular” in Ophryophryne elfina (Poyarkov et al. 2017), and O. microstoma Boulenger, 1903 (tadpoles described by Grosjean, 2003 without molecular data), but this rim is “serrated” in Boulenophrys tadpoles, described both in this study and in Tapley et al. (2020b); or the oral disc is “hastate shaped” in Atympanophrys gigantica (Tapley et al. 2020b), which has not been reported in other genera. We failed to find any Xenophrys mangshanensis adult or larva during our field surveys, despite Mangshan being its type locality. However, it was reported to be in sympatry with Bo. nanlingensis and Br. popei in Guangdong Province (Wang et al. 2019). As earlier mentioned under the ecology notes for Boulenophrys cf. ombrophila, sometimes larvae maybe washed downstream where adult frogs are not thought to be present. Therefore, if megophryinid species occur in sympatry with others, the tadpole identification without molecular data should be re-considered. Perhaps, the tadpole specimens previously described as X. mangshanensis, for example, by Fei and Ye (2016) should be re-examined after molecular identification. The presence of Bo. kuatunensis and Bo. brachykolos in Mangshan was not con- firmed in this study, which agrees with the conjecture proposed by Liu et al. (2018) and Tapley et al. (2017) that both species are restricted to their type localities. However, taxonomic revisions need adequate field surveys and detailed examination of museum specimens (Qi et al. 2021). This study provides an example of using tadpole identi- fication to document the presence of species where adults may not always be visible. Tadpoles of four megophryinid frogs from China LF This proved particularly useful here as the tadpoles of megophryinid frogs at this site appear to be relatively slow to develop, and they could always be found outside the breeding season. Acknowledgements We would like to thank Yuanlingbo Shang and Yao Luo for help collecting specimens; Guoxing Deng for assistance during field surveys. We thank Chaosheng Mu for the help in collecting literature. TQ thanks Zhengyan Zhou for the guidance in tadpole photography, and Yu Zhang for the help in tadpole staging. We are sincerely grateful to Annemarie Ohler, Benjamin Tapley, and an anonymous reviewer for their helpful comments and suggestions. 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